Regulatory
cI 434

Part:BBa_I12014

Designed by: ryhsiao   Group: Antiquity   (2004-07-21)


Repressor, 434 cI (RBS- LVA-)

The 434 cI repressor protein coding sequence is a 710 base-pair sequence with the standard RBS-compatible BioBrick prefix and the standard BioBrick suffix sections on its ends. It binds to the 434 regulatory sequence, BBa_R0052. The sequence has no LVA tag nor RBS.


Usage and Biology

434 cI is a member of the lamboid cI protein family. The coding sequence has been modified from wild type cI to contain two TAA stop codons, and its EcoRI recognition site has been mutated out by changing base 36 from T to C to be BioBrick-compatible.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/regulatory/uncategorized
Parameters
None